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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
13/11/2015 |
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Actualizado : |
13/11/2015 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
LADO, J.; CRONJE, P.; ALQUÉZAR, B.; PAGE, A.; MANZI, M.; GÓMEZ-CADENAS, A.; STEAD, A.D.; ZACARÍAS, L.; RODRIGO, M.J. |
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Afiliación : |
JOANNA LADO LINDNER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Fruit shading enhances peel color, carotenes accumulation and chromoplast differentiation in red grapefruit. |
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Fecha de publicación : |
2015 |
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Fuente / Imprenta : |
Physiologia Plantarum, 2015, v.154, no. 4, p. 469-484. |
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Serie : |
0031-9317 |
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DOI : |
10.1111/ppl.12332 |
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Idioma : |
Inglés |
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Contenido : |
ABSTRACT.
The distinctive color of red grapefruits is due to lycopene, an unusual carotene in citrus. It has been observed that red ?Star Ruby? (SR) grapefruits grown inside the tree canopy develop a more intense red coloration than those exposed to higher light intensities. To investigate the effect of light on SR peel pigmentation, fruit were bagged or exposed to normal photoperiodic conditions, and changes in carotenoids, expression of carotenoid biosynthetic genes and plastid ultrastructure in the peel were analyzed. Light avoidance accelerated chlorophyll breakdown and induced carotenoid accumulation, rendering fruits with an intense coloration. Remarkably, lycopene levels in the peel of shaded fruits were 49-fold higher than in light-exposed fruit while concentrations of downstream metabolites were notably reduced, suggesting a bottleneck at the lycopene cyclization in the biosynthetic pathway. Paradoxically, this increment in carotenoids in covered fruit was not mirrored by changes in mRNA levels of carotenogenic genes, which were mostly up-regulated by light. In addition, covered fruits experienced profound changes in chromoplast differentiation, and the relative expression of genes related to chromoplast
development was enhanced. Ultrastructural analysis of plastids revealed an acceleration of chloroplasts to chromoplast transition in the peel of covered fruits concomitantly with development of lycopene crystals and plastoglobuli. In this sense, an accelerated differentiation of chromoplasts may provide biosynthetic capacity and a sink for carotenoids without involving major changes in transcript levels of carotenogenic genes. Light signals seem to regulate carotenoid accumulation at the molecular and structural level by
influencing both biosynthetic capacity and sink strength. Abbreviations ? 𝛽CHX, 𝛽-carotene hydroxylase; 𝛽LCY, lycopene cyclase 𝛽; ABA, abscisic acid; C, covered; Chl, chlorophyll; DXS, 1-deoxy-D-xylulose-5-phosphate synthase; FIB, fibrillin; FW, fresh weight; GGPP, geranyl geranyl pyrophosphate; GGPPS, geranyl geranyl pyrophosphate synthase; HDR, hydroxymethylbutenyl diphosphate reductase; HPLC, high-performance liquid chromatography; MEP, methyl-D-erythritol-4-phosphate; NC, non-covered; PCR, polymerase chain reaction; PDS, phytoene desaturase; PSY, phytoene synthase; sHSP, small heat shock protein; SR, Star Ruby; ZDS, 𝜁-carotene desaturase.
Physiol. Plant. MenosABSTRACT.
The distinctive color of red grapefruits is due to lycopene, an unusual carotene in citrus. It has been observed that red ?Star Ruby? (SR) grapefruits grown inside the tree canopy develop a more intense red coloration than those exposed to higher light intensities. To investigate the effect of light on SR peel pigmentation, fruit were bagged or exposed to normal photoperiodic conditions, and changes in carotenoids, expression of carotenoid biosynthetic genes and plastid ultrastructure in the peel were analyzed. Light avoidance accelerated chlorophyll breakdown and induced carotenoid accumulation, rendering fruits with an intense coloration. Remarkably, lycopene levels in the peel of shaded fruits were 49-fold higher than in light-exposed fruit while concentrations of downstream metabolites were notably reduced, suggesting a bottleneck at the lycopene cyclization in the biosynthetic pathway. Paradoxically, this increment in carotenoids in covered fruit was not mirrored by changes in mRNA levels of carotenogenic genes, which were mostly up-regulated by light. In addition, covered fruits experienced profound changes in chromoplast differentiation, and the relative expression of genes related to chromoplast
development was enhanced. Ultrastructural analysis of plastids revealed an acceleration of chloroplasts to chromoplast transition in the peel of covered fruits concomitantly with development of lycopene crystals and plastoglobuli. In this sense, an accelerated diff... Presentar Todo |
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Thesagro : |
CITRUS; CITRUS PARADISI. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 03223naa a2200265 a 4500
001 1053867
005 2015-11-13
008 2015 bl uuuu u00u1 u #d
024 7 $a10.1111/ppl.12332$2DOI
100 1 $aLADO, J.
245 $aFruit shading enhances peel color, carotenes accumulation and chromoplast differentiation in red grapefruit.$h[electronic resource]
260 $c2015
490 $a0031-9317
520 $aABSTRACT. The distinctive color of red grapefruits is due to lycopene, an unusual carotene in citrus. It has been observed that red ?Star Ruby? (SR) grapefruits grown inside the tree canopy develop a more intense red coloration than those exposed to higher light intensities. To investigate the effect of light on SR peel pigmentation, fruit were bagged or exposed to normal photoperiodic conditions, and changes in carotenoids, expression of carotenoid biosynthetic genes and plastid ultrastructure in the peel were analyzed. Light avoidance accelerated chlorophyll breakdown and induced carotenoid accumulation, rendering fruits with an intense coloration. Remarkably, lycopene levels in the peel of shaded fruits were 49-fold higher than in light-exposed fruit while concentrations of downstream metabolites were notably reduced, suggesting a bottleneck at the lycopene cyclization in the biosynthetic pathway. Paradoxically, this increment in carotenoids in covered fruit was not mirrored by changes in mRNA levels of carotenogenic genes, which were mostly up-regulated by light. In addition, covered fruits experienced profound changes in chromoplast differentiation, and the relative expression of genes related to chromoplast development was enhanced. Ultrastructural analysis of plastids revealed an acceleration of chloroplasts to chromoplast transition in the peel of covered fruits concomitantly with development of lycopene crystals and plastoglobuli. In this sense, an accelerated differentiation of chromoplasts may provide biosynthetic capacity and a sink for carotenoids without involving major changes in transcript levels of carotenogenic genes. Light signals seem to regulate carotenoid accumulation at the molecular and structural level by influencing both biosynthetic capacity and sink strength. Abbreviations ? 𝛽CHX, 𝛽-carotene hydroxylase; 𝛽LCY, lycopene cyclase 𝛽; ABA, abscisic acid; C, covered; Chl, chlorophyll; DXS, 1-deoxy-D-xylulose-5-phosphate synthase; FIB, fibrillin; FW, fresh weight; GGPP, geranyl geranyl pyrophosphate; GGPPS, geranyl geranyl pyrophosphate synthase; HDR, hydroxymethylbutenyl diphosphate reductase; HPLC, high-performance liquid chromatography; MEP, methyl-D-erythritol-4-phosphate; NC, non-covered; PCR, polymerase chain reaction; PDS, phytoene desaturase; PSY, phytoene synthase; sHSP, small heat shock protein; SR, Star Ruby; ZDS, 𝜁-carotene desaturase. Physiol. Plant.
650 $aCITRUS
650 $aCITRUS PARADISI
700 1 $aCRONJE, P.
700 1 $aALQUÉZAR, B.
700 1 $aPAGE, A.
700 1 $aMANZI, M.
700 1 $aGÓMEZ-CADENAS, A.
700 1 $aSTEAD, A.D.
700 1 $aZACARÍAS, L.
700 1 $aRODRIGO, M.J.
773 $tPhysiologia Plantarum, 2015$gv.154, no. 4, p. 469-484.
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas; INIA Treinta y Tres. |
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Fecha actual : |
02/12/2019 |
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Actualizado : |
05/03/2020 |
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Tipo de producción científica : |
Serie FPTA |
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Autor : |
ROSAS, F.; ARBELETCHE, P.; MAZZILLI, S.; SILVA, M.E.; PELOCHE, D.; MONDELLI, M. |
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Afiliación : |
FRANCISCO ROSAS, Universidad ORT Uruguay; Centro de Investigaciones Económicas (CINVE); PEDRO ARBELETCHE; SEBASTIÁN R. MAZZILLI; MARÍA E. SILVA CARRAZZONE; DAIANA PELOCHE; MARIO MONDELLI. |
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Título : |
Cuantificación del impacto en el uso de recursos naturales y el medio ambiente de diversos sistemas productivos agrícolas por taxonomía organizacional. |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
Montevideo (UY): INIA, 2019. |
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Páginas : |
79 p. |
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Serie : |
(Serie FPTA-INIA; 77). |
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ISBN : |
978-9974-38-426-2 |
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ISSN : |
1688-924X |
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Idioma : |
Español |
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Notas : |
Proyecto FPTA 330: "Cuantificación del impacto en el uso de recursos naturales y el medio ambiente de diversos sistemas productivos agrícolas por tipología organizacional." Período de ejecución: Junio 2014 - Julio 2016. Institución Ejecutora: Centro de Investigaciones Económicas (CINVE). Responsable técnico del proyecto: Francisco Rosas. "Dedicado a la memoria del Ing. Agr. (PhD) Mario Mondelli". |
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Contenido : |
Propósito: Existen ciertos modelos organizacionales que promueven sistemas productivos que son más sustentables desde el punto de vista de la conservación de los
recursos naturales y medioambiente. Esas diferencias responden a incentivos diferentes en torno al uso y conservación de los recursos, y tienen impacto diferencial en atributos ambientales. |
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Thesagro : |
MEDIO AMBIENTE; RECURSOS NATURALES. |
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Asunto categoría : |
P01 Conservación de la naturaleza y recursos de La tierra |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/13866/1/Inia-Fpta-77-proyecto-330-2019.pdf
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Marc : |
LEADER 01491nam a2200253 a 4500
001 1060482
005 2020-03-05
008 2019 bl uuuu u00u1 u #d
020 $a978-9974-38-426-2
022 $a1688-924X
100 1 $aROSAS, F.
245 $aCuantificación del impacto en el uso de recursos naturales y el medio ambiente de diversos sistemas productivos agrícolas por taxonomía organizacional.
260 $aMontevideo (UY): INIA$c2019
300 $a79 p.
490 $a(Serie FPTA-INIA; 77).
500 $aProyecto FPTA 330: "Cuantificación del impacto en el uso de recursos naturales y el medio ambiente de diversos sistemas productivos agrícolas por tipología organizacional." Período de ejecución: Junio 2014 - Julio 2016. Institución Ejecutora: Centro de Investigaciones Económicas (CINVE). Responsable técnico del proyecto: Francisco Rosas. "Dedicado a la memoria del Ing. Agr. (PhD) Mario Mondelli".
520 $aPropósito: Existen ciertos modelos organizacionales que promueven sistemas productivos que son más sustentables desde el punto de vista de la conservación de los recursos naturales y medioambiente. Esas diferencias responden a incentivos diferentes en torno al uso y conservación de los recursos, y tienen impacto diferencial en atributos ambientales.
650 $aMEDIO AMBIENTE
650 $aRECURSOS NATURALES
700 1 $aARBELETCHE, P.
700 1 $aMAZZILLI, S.
700 1 $aSILVA, M.E.
700 1 $aPELOCHE, D.
700 1 $aMONDELLI, M.
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